Voltage-sensitive prestin orthologue expressed in zebrafish hair cells

Jörg T. Albert, Harald Winter, Thorsten J. Schaechinger, Thomas Weber, Xiang Wang, David Z. He, Oliver Hendrich, Hyun Soon Geisler, Ulrike Zimmermann, Katrin Oelmann, Marlies Knipper, Martin C. Göpfert, Dominik Oliver

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Prestin, a member of the solute carrier (SLC) family SLC26A, is the molecular motor that drives the somatic electromotility of mammalian outer hair cells (OHCs). Its closest reported homologue, zebrafish prestin (zprestin), shares ∼70% strong amino acid sequence similarity with mammalian prestin, predicting an almost identical protein structure. Immunohistochemical analysis now shows that zprestin is expressed in hair cells of the zebrafish ear. Similar to mammalian prestin, heterologously expressed zprestin is found to generate voltage-dependent charge movements, giving rise to a non-linear capacitance (NLC) of the cell membrane. Compared with mammalian prestin, charge movements mediated by zprestin display a weaker voltage dependence and slower kinetics; they occur at more positive membrane voltages, and are not associated with electromotile responses. Given this functional dissociation of NLC and electromotility and the structural similarity with mammalian prestin, we anticipate that zprestin provides a valuable tool for tracing the molecular and evolutionary bases of prestin motor function. copy; 2007 The Authors. Journal compilation

Original languageEnglish
Pages (from-to)451-461
Number of pages11
JournalJournal of Physiology
Volume580
Issue number2
DOIs
StatePublished - Apr 15 2007

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Zebrafish
Outer Auditory Hair Cells
Ear
Amino Acid Sequence
Cell Membrane
Membranes
Proteins

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Albert, J. T., Winter, H., Schaechinger, T. J., Weber, T., Wang, X., He, D. Z., ... Oliver, D. (2007). Voltage-sensitive prestin orthologue expressed in zebrafish hair cells. Journal of Physiology, 580(2), 451-461. https://doi.org/10.1113/jphysiol.2007.127993

Voltage-sensitive prestin orthologue expressed in zebrafish hair cells. / Albert, Jörg T.; Winter, Harald; Schaechinger, Thorsten J.; Weber, Thomas; Wang, Xiang; He, David Z.; Hendrich, Oliver; Geisler, Hyun Soon; Zimmermann, Ulrike; Oelmann, Katrin; Knipper, Marlies; Göpfert, Martin C.; Oliver, Dominik.

In: Journal of Physiology, Vol. 580, No. 2, 15.04.2007, p. 451-461.

Research output: Contribution to journalArticle

Albert, JT, Winter, H, Schaechinger, TJ, Weber, T, Wang, X, He, DZ, Hendrich, O, Geisler, HS, Zimmermann, U, Oelmann, K, Knipper, M, Göpfert, MC & Oliver, D 2007, 'Voltage-sensitive prestin orthologue expressed in zebrafish hair cells', Journal of Physiology, vol. 580, no. 2, pp. 451-461. https://doi.org/10.1113/jphysiol.2007.127993
Albert, Jörg T. ; Winter, Harald ; Schaechinger, Thorsten J. ; Weber, Thomas ; Wang, Xiang ; He, David Z. ; Hendrich, Oliver ; Geisler, Hyun Soon ; Zimmermann, Ulrike ; Oelmann, Katrin ; Knipper, Marlies ; Göpfert, Martin C. ; Oliver, Dominik. / Voltage-sensitive prestin orthologue expressed in zebrafish hair cells. In: Journal of Physiology. 2007 ; Vol. 580, No. 2. pp. 451-461.
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