Zn2+ inhibits the anion transport activity of Band 3 by binding to its cytoplasmic tail

Y. P. Tu; H. Xu

doi:10.1007/BF01200245

Zn²⁺ inhibits the anion transport activity of Band 3 by binding to its cytoplasmic tail

Y. P. Tu, H. Xu

Research output: Contribution to journal › Article

10 Scopus citations

Abstract

Zn²⁺ can induce a conformational change of Band 3 with concomitant inhibition of its anion transport activity of human erythrocyte membrane vesicles only from the cytoplasmic side. The Zn²⁺ inhibition exhibits a dose-dependent manner with an apparent half maximal concentration of 50 μM ZnCl₂ and can be reversed by 0.5 mM EDTA, but not by 1 mM dithiothreitol. The Zn²⁺ effect is specific and no similar inhibitory action could be observed by other divalent cations (Cu²⁺, Mn²⁺, Mg²⁺ or Sr²⁺).

Original language	English (US)
Pages (from-to)	159-169
Number of pages	11
Journal	Bioscience Reports
Volume	14
Issue number	4
DOIs	https://doi.org/10.1007/BF01200245
State	Published - Aug 1 1994
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1007/BF01200245

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Tu, Y. P., & Xu, H. (1994). Zn²⁺ inhibits the anion transport activity of Band 3 by binding to its cytoplasmic tail. Bioscience Reports, 14(4), 159-169. https://doi.org/10.1007/BF01200245

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abstract = "Zn2+ can induce a conformational change of Band 3 with concomitant inhibition of its anion transport activity of human erythrocyte membrane vesicles only from the cytoplasmic side. The Zn2+ inhibition exhibits a dose-dependent manner with an apparent half maximal concentration of 50 μM ZnCl2 and can be reversed by 0.5 mM EDTA, but not by 1 mM dithiothreitol. The Zn2+ effect is specific and no similar inhibitory action could be observed by other divalent cations (Cu2+, Mn2+, Mg2+ or Sr2+).",

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N2 - Zn2+ can induce a conformational change of Band 3 with concomitant inhibition of its anion transport activity of human erythrocyte membrane vesicles only from the cytoplasmic side. The Zn2+ inhibition exhibits a dose-dependent manner with an apparent half maximal concentration of 50 μM ZnCl2 and can be reversed by 0.5 mM EDTA, but not by 1 mM dithiothreitol. The Zn2+ effect is specific and no similar inhibitory action could be observed by other divalent cations (Cu2+, Mn2+, Mg2+ or Sr2+).

AB - Zn2+ can induce a conformational change of Band 3 with concomitant inhibition of its anion transport activity of human erythrocyte membrane vesicles only from the cytoplasmic side. The Zn2+ inhibition exhibits a dose-dependent manner with an apparent half maximal concentration of 50 μM ZnCl2 and can be reversed by 0.5 mM EDTA, but not by 1 mM dithiothreitol. The Zn2+ effect is specific and no similar inhibitory action could be observed by other divalent cations (Cu2+, Mn2+, Mg2+ or Sr2+).

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